Spray down workspace with bleach solution. pGLO Transformation & Purification summary LAB Name_ _ _ _ _ Students use a simple procedure pglo to transform bacteria with a gene that codes for pglo Green Fluorescent Protein ( GFP). Demonstrate the power of genetic summary transformation! Remove the rack containing the tubes from the ice and pglo place on the lab counter. Complete sheet the remainder of the activity sheet you pglo began on day summary sheet 1 of the lab. Introduction to Biotechnology Unit: Students will demonstrate knowledge of biotechnology concepts and summary apply knowledge to real- world problems in inquiry- based laboratories. The plasmid contains several reporter genes most notably for the green fluorescent protein ( GFP) the ampicillin resistance gene.
Ideal for sheet AP Biology Lab 6. Repeat with a new micropipette tip for the – pGLO tube. Students will glow with excitement when they transform bacteria with pGLO plasmid. The pGLO plasmid is an engineered plasmid used in biotechnology as a vector pglo for creating genetically modified organisms. ” A gene is a piece of DNA that provides the instructions for making ( summary it codes for sheet . With a L lab micropipette with a sterile tip, transfer 250 L of LB nutrient broth to the + pGLO summary tube.
Michelle Landreville Biotechnology Unit 1: The lab techniques and analysis of the pglo data. Open the petri plates and immerse in the sheet large container of bleach solution your instructor has provided. Pglo lab summary sheet. Close the tube and gently tap with your finger to mix. Emphasis will be on lab technique and analysis of the data. GFP was isolated from the jelly fish Aequorea victoria.
The mutant form used in the pGLO plasmid is called the cycle 3 mutant and has three point mutations: phenylalanine100, methionine154, and valine164, which were mutated to serine, threonine, and alanine. The complete amino acid sequence of the cycle 3 mutant is shown in Fig. 4 ( Crameri et al. Start studying Spring Summary Sheet. Learn vocabulary, terms, and more with flashcards, games, and other study tools.
pglo lab summary sheet
Biology of the Cell Lab ( BIOLTransformation Lab Worksheet. Lab 9 - Transformation Worksheet Author: Biological Sciences Last modified by:.